Boao Initiative of the 2025 Asia Pacific Congress on Public Health

The World Federation of Public Health Associations (WFPHA) Asia Pacific Regional Liaison Office,The Chinese Preventive Medicine Association (CPMA)

Prepared by:

Xiaonong Zhou^1,2; Minghui Ren^3,4

1. National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention (Chinese Center for Tropical Diseases Research); NHC Key Laboratory of Parasite and Vector Biology; WHO Collaborating Centre for Tropical Diseases; National Center for International Research on Tropical Diseases, Ministry of Science and Technology,Shanghai, China;

2. School of Global Health, Chinese Center for Tropical Diseases Research, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

3. China Centre for Health Development Studies, Peking University, Beijing, China;

4. Department of Global Health, School of Public Health, Peking University, Beijing, China.

The 2025 Asia Pacific Congress on Public Health, held in Boao, China, in November 2025, released a transnational consensus and jointly issued the Boao Initiative by Chinese Preventive Medicine Association, World Federation of Public Health Associations, and other six associations from the Asia Pacific region. Centered on the core themes of equity, resilience, and innovation, the Initiative proposes a “Four-in-One” collaborative action plan to address the Asia Pacific region’s severe public health challenges. These challenges include fragmented regional public health governance, widening health inequalities, increasing climate-health risks, and structural constraints on digital transformation. To tackle these issues, the “Four-in-One” plan includes four key components: 1) Strengthening regional governance and building joint prevention and control defenses; 2) Advancing health equity and protecting every life; 3) Enhancing health adaptation to climate change and building a resilient barrier; and 4) Leading digital innovation and empowering smart health systems. The initiative underscores the importance of transnational cooperation to build a comprehensive, inclusive, and sustainable public health framework. It reaffirms the Asia Pacific region’s commitment to global health governance, advocating for shared responsibility to overcome disparities and foster resilience through innovation. Therefore, the Boao Initiative serves as a strategic roadmap for achieving equitable health outcomes and strengthening regional collaboration in public health.

2025亚太公共卫生大会博鳌倡议

世界公共卫生协会联盟亚太地区办公室；中华预防医学会。

执笔人：

周晓农^1,2；任明辉^3,4

1. 寄生虫病预防控制所，中国疾病预防控制中心(国家热带病研究中心); 国家卫生健康委员会寄生虫与媒介生物学重点实验室; 世界卫生组织热带病合作中心; 国家科技部热带病国际研究中心，上海市，中国；

2. 全球健康学院，中国热带病研究中心，上海交通大学医学院，上海市，中国。

3. 健康发育研究中心，北京大学，北京市，中国；

4. 全球健康系，公共卫生学院，北京大学，北京市，中国。

2025年11月，2025年亚太公共卫生大会在中国博鳌召开。会上，由中华预防医学会、世界公共卫生协会联盟及其他亚太地区的六个协会共同发布了跨国共识，并联合发起了《博鳌倡议》。该倡议以“公平、韧性、创新”为核心主题，提出了一项“四位一体”协同行动计划，旨在应对亚太地区严峻的公共卫生挑战。这些挑战包括区域公共卫生治理碎片化、区域内健康不平等加剧、气候健康风险日益增加以及数字化转型面临的结构性制约。为应对这些问题，“四位一体”计划包含四个关键组成部分：1）加强区域治理，构建联防联控防线；2）促进健康公平，守护每一个生命；3）增强健康对气候变化的适应性，构建韧性屏障；4）引领数字创新，赋能智慧健康体系。该倡议强调了跨国合作对于构建一个全面、包容、可持续的公共卫生框架的重要性。它重申了亚太地区对全球卫生治理的承诺，倡导通过共担责任来消除差距，并通过创新培育韧性。因此，《博鳌倡议》是一份战略路线图，为实现公平的健康成果和加强公共卫生领域的区域协作指明了方向。

For more information: https://weekly.chinacdc.cn/en/article/doi/10.46234/ccdcw2025.259

Outbreak of Chikungunya Virus with Aedes albopictus-Adaptive Mutations — Guangdong Province, China, 2025

Wenxiao Gong¹; Dongli Wang¹; Qianying Chen¹; Shuting Zhong¹; Xiaolu Shi^{2, 3#}; Bo Peng^1,2,#

1. Center for Disease Control and Prevention of Guangming District, Shenzhen City, Guangdong Province, China;
2. Microbiology Laboratory, Shenzhen Center for Disease Control and Prevention, Shenzhen City, Guangdong Province, China;
3. School of Public Health, Southern Medical University, Guangzhou City, Guangdong Province, China.

^# Corresponding author: Bo Peng: pb18@163.com; Xiaolu Shi: shixiaolu831@163.com.

Chikungunya virus (CHIKV), transmitted by Aedes aegypti and Aedes albopictus, causes chikungunya fever. Since its 2005 re-emergence, it has become endemic in 119 countries across Africa, Asia, the Americas, Europe, and Oceania. An outbreak in China’s Guangdong Province (July 2025) led to about 15,000 cases by August 20, straining public health systems and highlighting the need to strengthen viral mutation surveillance. However, the precise genomic characteristics of this prevalent virus remain unknown, and this study aims to fill this critical knowledge gap through high-throughput sequencing technology. This study collected two serum samples from this epidemic, extracting and sequencing total nucleic acids via an MGISEQ-G99 sequencer. Complete viral genomes were generated via a consensus reference assembly; they were phylogenetically analyzed and comparatively assessed to identify viral nucleotide variations and protein amino acid substitutions. Phylogenetic analysis confirmed that both research strains belong to the East/Central/South African genotype, clustering within the Middle African Lineage and sharing the highest identity with currently circulating CHIKV isolates from Réunion Island. The circulating strain carries adaptive mutation sites such as E1-A226V, E2-L210Q, and E2-I211T, significantly enhancing viral replication efficiency in Ae. albopictus. Understanding viral etiology is essential for controlling outbreaks. The current epidemic strain carries mutations adaptive to Ae. albopictus, increasing transmission risk. Guangdong’s mosquito population — predominantly Ae. albopictus with limited Ae. aegypti presence—facilitates efficient virus import and spread.

基孔肯雅热爆发疫情流行毒株携带白纹伊蚊适应性突变 — 广东省，中国， 2025年

龚文孝¹，汪东篱¹，陈倩盈¹，钟淑婷¹，石晓路^2,3,#，彭博^1,2,#

1. 深圳市光明区疾病预防控制中心，深圳市，广东省，中国；

2. 病原微生物检验所，深圳市疾病预防控制中心，深圳市，广东省，中国；

3. 公共卫生学院，南方医科大学，广州市，广东省，中国。

^# 通信作者: 彭博：pb18@163.com；石晓路：shixiaolu831@163.com。

基孔肯雅病毒（CHIKV）是一种通过埃及伊蚊和白纹伊蚊传播的病原体，可引起基孔肯雅热。自2005年再度出现以来，该病毒已在非洲、亚洲、美洲、欧洲和大洋洲等地区的119个国家形成地方性流行。2025年7月中国广东省暴发疫情，截至8月20日已导致约1.5万例感染，对公共卫生系统造成压力，凸显出加强病毒突变监测的紧迫性。本次疫情中采集到2份基孔肯雅病毒阳性血清样本。提取总核酸后使用MGISEQ-G99测序仪（华大智造）进行测序，通过参考序列比对后组装获得完整病毒基因组。对基因组进行系统发育分析及比对评估，以识别病毒核苷酸变异及蛋白氨基酸替换位点。系统发育分析证实本研究中的毒均属于东/中/南非基因型，特异性聚类于中非谱系，与当前留尼汪岛流行的CHIKV分离株同源性最高。研究进一步确认流行毒株携带E1-A226V、E2-L210Q和E2-I211T等适应性突变，这些位点已被证实可显著增强病毒在白纹伊蚊体内的复制效率。病毒基因组变异特征测对疫情控制至关重要。当前流行株携带适应白纹伊蚊的突变位点，增加了传播风险。广东省蚊媒种群以白纹伊蚊为主，埃及伊蚊分布有限，这种生态特征为病毒的高效输入和传播创造了条件。

For more information: https://weekly.chinacdc.cn/en/article/doi/10.46234/ccdcw2025.260

The First Imported Case of Chikungunya Virus Infection — Anhui Province, China, 2025

Liangzi Guo^1,&, Jun He^2,&, Sai Hou³, Ziqing Yuan⁴, Junling Yu³, Zhaoqian Meng¹, Guoxia Guo¹, Xialian Gao¹, Huaibiao Li¹, Lei Gong³, Yinxu Hou³, Jialin Zhang³, Xiuzhi Chen³, Yujie Feng³, Xiaofeng Lyu³, Tuantuan Li⁴, Ge Bu^1,#, Jiabing Wu^3,#

1. Fuyang Center for Disease Control and Prevention, Fuyang City, Anhui Province, China;

2. Department of Microbiology Laboratory, Anhui Provincial Academy of Preventive Medicine, Hefei City, Anhui Province, China;

3. Anhui Provincial Center for Disease Control and Prevention, Hefei City, Anhui Province, China;

4. Fuyang Second People's Hospital, Fuyang City, Anhui Province, China.

^& Joint first authors.

^# Corresponding author: Jiabing Wu, wjb0386@ahcdc.com.cn; Ge Bu, fycdcbuge@163.com.

On August 11, 2025, a suspected Chikungunya virus (CHIKV) case traveled from Foshan to Fuyang Xiguan Airport, Anhui. After symptom reporting on August 12, local CDC launched epidemiological investigations, laboratory testing, and control measures. We collected serial blood samples throughout the patient's hospitalization and documented the complete clinical progression from admission to discharge. Real-time fluorescent quantitative PCR (qPCR) was employed to detect CHIKV nucleic acid, while Enzyme-Linked Immunosorbent Assay (ELISA) was used to test for CHIKV IgG and IgM antibodies. MinION nanopore sequencing was performed on blood samples to obtain the complete viral genome sequence. Phylogenetic analyses were subsequently constructed to determine the origin, genotype, and mutation profile of this imported case. The qPCR analysis confirmed CHIKV presence in the patient's serum samples. ELISA detected CHIKV IgG and IgM antibodies in blood samples collected on the fifth and ninth days after illness onset, respectively. Nanopore sequencing successfully generated the complete CHIKV genome sequence. Phylogenetic analysis demonstrated that the strain belonged to the East-Central-South-African lineage, consistent with the genotype identified from 190 cases in the local clustered Chikungunya fever (CHIKF) outbreak that occurred in Guangdong Province in July 2025. The strain showed 99.964% nucleotide identity (4 differential loci) with strain PX216392.1 detected in the current epidemic, and 99.9556% identity (5 differential loci) with PX236189.1. This is Anhui’s first imported CHIKV case, linked to the Guangdong outbreak. No local transmission or CHIKV-positive vector mosquitoes were detected.

首例输入性基孔肯雅热病毒感染 — 安徽省，中国，2025年

郭靓子^1,&；何军^2,&；侯赛³；袁子清⁴；俞俊岭³；孟昭倩¹；郭国侠¹；高夏莲¹；李淮彪¹；龚磊³；侯银续³；张家林³；陈秀芝³；冯玉杰³；吕晓凤³；李团团⁴；卜戈^1,#；吴家兵^2,#

1. 阜阳市疾病预防控制中心，阜阳市，安徽省，中国；

2. 微生物学实验室，安徽省预防医学科学院，合肥市，安徽省，中国；

3. 安徽省疾病预防控制中心，合肥市，安徽省，中国；

4. 阜阳市第二人民医院，阜阳市，安徽省，中国。

^& 共同第一作者。

^# 通信作者：吴家兵，wjb0386@ahcdc.com.cn; 卜戈，fycdcbuge@163.com。

通过分析安徽省首例输入性基孔肯雅热病例的发现、诊断、基因组特征分析以及疫情处置经过，为输入性基孔肯雅热防控工作提供参考，也为今后类似虫媒疫情的防控措施提供数据及经验支持。连续采集患者住院期间的血液标本，记录临床检测数据；用实时荧光定量PCR方法检测基孔肯雅病毒核酸；使用酶联免疫吸附试验（ELISA）检测基孔肯雅病毒的IgG和IgM抗体；扩增病毒全基因组并测序，使用生物信息学软件对病毒株的全基因组遗传特征进行分析；患者确诊后立即开展流行病学调查、媒介伊蚊应急监测和处置。经检测确认，该患者基孔肯雅病毒核酸阳性，为安徽省首例基孔肯雅热病例。特异性引物扩增获得序列表明该毒株属于东中南部非洲谱系，与2025年7月广东省本地聚集性基孔肯雅热（CHIKF）疫情中检测到的PX216392.1毒株在4个差异位点上一致性为99.964%，与PX236189.1毒株在5个差异位点上一致性为99.9556%。本次疫情未引起本地扩散。

For more information: https://weekly.chinacdc.cn/en/article/doi/10.46234/ccdcw2025.261